Assessing genetic diversity of promising wheat (Triticum aestivum L.) lines using microsatellite markers linked with salinity tolerance

نویسندگان

  • Ghasem Mohammadi Nejad Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Bahonar University of Kerman
چکیده مقاله:

Narrow genetic variability may lead to genetic vulnerability of field crops against biotic and abiotic stresses which can cause yield reduction. In this study a set of 37 wheat microsatellite markers linked with identified QTLs for salinity tolerance were used for the assessment of genetic diversity for salinity in 30 promising lines of hexaploid bread wheat (Triticum aestivum L.). A total of 438 alleles were detected with an average allele number of 11.84 per locus using 37 microsatellite markers. The number of alleles per locus ranged from two to twenty, the maximum number of alleles was observed at Xgwm312. Gene diversity statistic for 37 microsatellite loci was varied from 0.66 to 0.94 and also polymorphic information content value was varied from 0.64 to 0.93 for Xgwm445 and Xgwm312 respectively. Result showed Xgwm312 SSR marker with the highest PIC value was distinguished as the best marker for genetic diversity analysis to improve of salinity tolerance. Obtained dendrogram by UPGMA method categorized genotypes in to 3 different groups, which had different reaction to salinity. A wide range of genomic diversity was observed among all the genotypes. Principal Coordinates Analysis (PCoA) also confirmed this pattern of genetic diversity, proving them can be use as the prime candidates in order to improve of salinity tolerance in breeding programs of wheat. The present study also indicates that microsatellite markers permit the fast and high throughput fingerprinting of numbers of genotypes from a germplasm collection in order to assess genetic diversity.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers

In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (PIC) varied from 0.66 (Xgwm429) to 0.94 (Xgwm212 and Xgw...

متن کامل

Assessment of Genetic Diversity of Bread Wheat (Triticum aestivum L.) Cultivars using Microsatellite Markers

Determining the level of genetic diversity and relationships among genotypes is foundation for identifying the appropriate parents in different breeding objects. In present study, the genetic diversity of 22 wheat cultivars were tested using 22 pairs of SSR primers. Out of 24 detected alleles, 11 were able to show desirable polymorphism. In average 2.18 alleles per locus were generated, in whic...

متن کامل

assessment of genetic diversity in iranian wheat (triticum aestivum l.) cultivars and lines using microsatellite markers

in this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (ssr) alleles, covering all three wheat genomes. a total of 1557 allelic variants were detected for 126 ssr loci. the number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (pic) varied from 0.66 (xgwm429) to 0.94 (xgwm212 and xgwm515). the high­est po...

متن کامل

Potassium induced salinity tolerance in wheat (Triticum aestivum L.)

Water culture experiments were conducted to study the response of ten wheat genotypes to external K application (10 mmol KCI dm3) at seedling stage under saline condition (0 and 100 mmol NaCl dm3). The data showed that there was an increase in the shoot and root length with the application of external K. The increase was more pronounced under control than under saline conditions. The better per...

متن کامل

Genetic Diversity of Bread Wheat (Triticum aestivum L.) Genotypes Using RAPD and ISSR Molecular Markers

The importance of grain cultivation especially wheat is obvious in terms of providing human and animal food and its impact on the economy of human societies. The reduction of genetic diversity in cultivars prevents increasing yields in line with rising demand and consumption. Therefore, it is necessary to improve the compatibility of them and increase their genetic extent. In the current, the g...

متن کامل

Study of Genetic Diversity in Wheat (triticum Aestivum L.) Using Random Amplified Polymorphic Dna (rapd) Markers

Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 1  شماره 2

صفحات  28- 39

تاریخ انتشار 2013-10-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023